By George Klein, Sidney Weinhouse, Alexander Haddow (Eds.)

ISBN-10: 0120066157

ISBN-13: 9780120066155

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Extra info for Advances in Cancer Research, Vol. 15

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Recently, Klietmann and Seemayer (1971), using the mixed hemadsorption test, showed marked differences in the concentration of specific surface antigen(s) on hamster cells transformed by normal SV40 and those transformed by UV-irradiated SV40. UV-irradiated virus also had a reduced capacity to induce SV40 transplantation immunity in vivo. The cells which had lower concentrations of surface antigen(s) as measured by the mixed hemadsorption test showed equal rates of growth in intraperitoneally implanted diffusion chambers in either immune or normal hamsters.

It would also strengthen the postulated rnechanism described above of the interferon resistance ( a ) of T-antigen synthesis in transformed cells and ( b ) of transformation after S phase growth of SV40-infected 3T3 cells. Evidence for such polycistronic 40 J. S. BUTEL, 6 . S. TEVETHIA, AND J . L.

Martin and Axelrod, 1969). A series of SV40-transformed mouse cell lines were analyzed. The extent of transcription in the individual lines varied, ranging from 30 to 100% of that seen during lytic infection. In the latter cell line, there must be some block beyond the point of transcription which prevents the synthesis of infectious virus. This study also emphasized that the extent of transcription of the SV40 genome is variable from one transformed cell line to the next, even within a single species of host cell.

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Advances in Cancer Research, Vol. 15 by George Klein, Sidney Weinhouse, Alexander Haddow (Eds.)

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